yxaA

168

putative glycerate kinase

locus: BSU_40040

Molecular weight: 39.24 kDa

pI: 4.53

Protein length: 382 aa

Gene length: 1149 bp

function: unknown

product: putative glycerate kinase

essential: no

ec: 2.7.1.31

synonyms: yxaA, glxK

List of homologs in different organisms, belongs to COG1929 (Galperin et al., 2021)

Gene

Coordinates: 4,112,073  4,113,221

The protein

Catalyzed reaction/ biological activity

• (R)-glycerate + ATP --> 3-phospho-D-glycerate + ADP + H⁺ (according to UniProt)

Protein family

• glycerate kinase type-1 family (single member, according to UniProt)

Structure

• [PDB|3CWC] (from Salmonella Typhimurium 58% identity)
• [AF|P42100]

Expression and Regulation

Operons

• genes: [gene|4907D4585491349E2592B0EF9150312A80785EE5|yxaA]-[gene|9A223AE85131511B7EA3A71EA57D87E8B167FA67|yxaB]

  description: [Pubmed|15805528,10746760]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|15805528,18326573], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15805528], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-B680 (yxaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1679 NBRP B. subtilis, Japan]
• BKE40040 ([gene|4907D4585491349E2592B0EF9150312A80785EE5|yxaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCAAATCGCCTCTTTCA,  downstream forward: _UP4_TAGCCGATGCACATGCCAAT
• BKK40040 ([gene|4907D4585491349E2592B0EF9150312A80785EE5|yxaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCAAATCGCCTCTTTCA,  downstream forward: _UP4_TAGCCGATGCACATGCCAAT

References

• 10746760