Difference between revisions of "CitG"
| Line 80: | Line 80: | ||
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1YFE 1YFE] (''E.coli'') | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1YFE 1YFE] (''E.coli'') | ||
| − | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/P07343 P07343] |
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33040] | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33040] | ||
| − | * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.2 4.2.1.2] | + | * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.2 4.2.1.2] |
=== Additional information=== | === Additional information=== | ||
Revision as of 14:28, 20 July 2009
- Description: fumarase
| Gene name | citG |
| Synonyms | |
| Essential | no |
| Product | fumarate hydratase |
| Function | TCA cycle |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 50 kDa, 5.475 |
| Gene length, protein length | 1386 bp, 462 aa |
| Immediate neighbours | gerAA, yuxN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU33040
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
- Protein family: Fumarase subfamily (according to Swiss-Prot)
- Paralogous protein(s): AnsB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 1YFE (E.coli)
- UniProt: P07343
- KEGG entry: [3]
- E.C. number: 4.2.1.2
Additional information
Expression and regulation
- Operon: citG
- Regulation: constitutive
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP718 (spec), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
