AcoL

• Description: acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
  
  

Gene name	acoL
Synonyms	yfjH
Essential	no
Product	acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Function	acetoin utilization
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	48 kDa, 5.273
Gene length, protein length	1374 bp, 458 aa
Immediate neighbours	acoC, acoR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU08090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD⁺ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

• Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: O34324

• KEGG entry: [3]

• E.C. number: 1.8.1.4

Expression and regulation

• Operon: acoA-acoB-acoC-acoL PubMed

• Sigma factor: SigL PubMed

• Regulation: repressed by glucose (CcpA) , induced by acetoin (AcoR) PubMed

• Regulatory mechanism: CcpA: transcription repression, AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

N O Ali, J Bignon, G Rapoport, M Debarbouille
Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis.
J Bacteriol: 2001, 183(8);2497-504
[PubMed:11274109] [WorldCat.org] [DOI] (P p)

M Huang, F B Oppermann-Sanio, A Steinbüchel
Biochemical and molecular characterization of the Bacillus subtilis acetoin catabolic pathway.
J Bacteriol: 1999, 181(12);3837-41
[PubMed:10368162] [WorldCat.org] [DOI] (P p)