SpoIIAB

• Description: anti-SigF/ protein serine kinase
  
  

Gene name	spoIIAB
Synonyms
Essential	no
Product	anti-SigF/ protein serine kinase
Function	control of SigF activity septation; phosphorylation and inactivation of SpoIIAA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	16 kDa, 4.383
Gene length, protein length	438 bp, 146 aa
Immediate neighbours	sigF, spoIIAA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)

• Protein family: anti-sigma-factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SpoIIE-SpoIIAB, RsbV-SpoIIAB, SpoIIAB-SigG, SpoIIAB-SigF, SpoIIAB-SpoIIAA

• Localization:

Database entries

• Structure: 1L0O (complex with SigF, Geobacillus stearothermophilus), 1TIL (complex with SpoIIAA, Geobacillus stearothermophilus)

• Swiss prot entry: P10728

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dacF-spoIIAA-spoIIAB-sigF

spoIIAA-spoIIAB-sigF

• Sigma factor: dacF: SigF, SigG

spoIIA: SigH

• Regulation: dacF: expressed during sporulation, spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism: spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J Bacteriol: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

K York, T J Kenney, S Satola, C P Moran, H Poth, P Youngman
Spo0A controls the sigma A-dependent activation of Bacillus subtilis sporulation-specific transcription unit spoIIE.
J Bacteriol: 1992, 174(8);2648-58
[PubMed:1556084] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed