dacF

168

Class C penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

Locus: BSU_23480

Molecular weight: 43.13 kDa

Isoelectric point: 9.91

Protein length: 389 aa

Gene length: 1170 bp

Function: control of peptide cross-linking in spore peptidoglycan

Product: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

Essential: no

Synonyms: dacF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)

Gene

Coordinates: 2,445,094 → 2,446,263

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth and colony morphology PubMed

The protein

Catalyzed reaction/ biological activity

• modifies degree of cross-linking of glycan strands in peptidoglycan PubMed
• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

• Peptidase S11 family (according to UniProt)

Structure

• 4K91 (PDB) (from Pseudomonas aeruginosa, 38% identity) PubMed
• P38422 (AlphaFold)

Paralogous protein(s)

• DacA, DacB

Localization

• secreted (via signal peptide) (according to UniProt)

Expression and Regulation

Operons

• Genes: dacF-spoIIAA-spoIIAB-sigF

  Description: PubMed

  Regulation

  • ''spoIIAA'': expressed early during sporulation

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigF: sigma factor, PubMed, in sigF regulon
  • SigG: sigma factor, in sigG regulon

Biological materials

Mutant

• BKE23480 (ΔdacF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT,  downstream forward: _UP4_TAATTATGCCGAATGACCAC
• BKK23480 (ΔdacF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT,  downstream forward: _UP4_TAATTATGCCGAATGACCAC

References

Reviews

Loading

Original Publications

Loading