Difference between revisions of "CysK"

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* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed], repressed in the presence of cysteine [[CymR]]
 
* '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed], repressed in the presence of cysteine [[CymR]]
 +
** repressed in the presence of cysteine ([[CymR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed]
  
 
* '''Regulatory mechanism:''' [[CymR]]: transcription repression
 
* '''Regulatory mechanism:''' [[CymR]]: transcription repression
 +
** [[CymR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 16:33, 11 June 2009

  • Description: trigger enzyme: cysteine synthetase A and control of CymR activity

Gene name cysK
Synonyms
Essential no
Product cysteine synthetase A
Function biosynthesis of cysteine, control of CymR activity
MW, pI 32 kDa, 5.492
Gene length, protein length 924 bp, 308 aa
Immediate neighbours yacD, pabB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CysK context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU00730

Phenotypes of a mutant

constitutive expression of the cysH-cysP-sat-cysC-ylnD-ylnE-ylnF operon, auxotrophic for cysteine

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + H2S = L-cysteine + acetate (according to Swiss-Prot)
  • Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by casamino acids PubMed, repressed in the presence of cysteine CymR
    • repressed in the presence of cysteine (CymR) PubMed
  • Regulatory mechanism: CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis.J. Biol. Chem. 283: 35551-35560. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed