Difference between revisions of "HrcA"

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(Basic information/ Evolution)
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
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* '''Catalyzed reaction/ biological activity:''' transcription repressor of the ''[[hrcA]]-[[grpE]]-[[dnaK]]-[[dnaJ]]'' and ''[[groES]]-[[groEL]]'' operons (at low/ normal temperatures)
  
 
* '''Protein family:'''
 
* '''Protein family:'''

Revision as of 16:28, 16 May 2009

  • Description: transcriptional repressor of class I heat-shock genes

Gene name hrcA
Synonyms yqxE, yqeS
Essential no
Product transcriptional repressor
Function regulation of chaperone gene expression
MW, pI 38 kDa, 6.079
Gene length, protein length 1029 bp, 343 aa
Immediate neighbours grpE, hemN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
HrcA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription repressor of the hrcA-grpE-dnaK-dnaJ and groES-groEL operons (at low/ normal temperatures)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by heat shock PubMed
  • Regulatory mechanism: repressed at normal temperature by HrcA
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

Your additional remarks

References

  1. Wetzstein, M., U. Völker, J. Dedio, S. Löbau, U. Zuber, M. Schiesswohl, C. Herget, M. Hecker, and W. Schumann. 1992. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J. Bacteriol. 174:3300-3310. PubMed
  2. Mogk, A., Homuth, G., Scholz, C., Kim, L., Schmid, F. X., and Schumann, W. (1997) The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis. EMBO J 16, 4579-4590. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed