Difference between revisions of "Sandbox"

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* '''Description:''' unknown <br/><br/>
+
* '''Description:''' small acid-soluble spore protein (minor) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''veg''
+
|''sspF''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || small acid-soluble spore protein (minor)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || protection of spore DNA
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 9 kDa, 4.814  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 9.647  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 258 bp, 86 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 183 bp, 61 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yabG]]'', ''[[sspF]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[veg]]'', ''[[ispE]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11820&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11821&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:veg_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:sspF_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/veg.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sspF.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10107]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10108]
  
 
=== Additional information===
 
=== Additional information===
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37466 P37466]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37549 P37549]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00440]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00450]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''veg''
+
* '''Operon:''' ''sspF'' [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:''' [[SigG]] [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:''' switched on during stage  III of sporulation [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
Line 118: Line 118:
 
=References=
 
=References=
  
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
# Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in ''Bacillus subtilis''. ''J. Mol. Biol.'' '''176:''' 333-348. [http://www.ncbi.nlm.nih.gov/sites/entrez/6205155 PubMed]

Revision as of 15:27, 4 May 2009

  • Description: small acid-soluble spore protein (minor)

Gene name sspF
Synonyms
Essential no
Product small acid-soluble spore protein (minor)
Function protection of spore DNA
MW, pI 6 kDa, 9.647
Gene length, protein length 183 bp, 61 aa
Immediate neighbours veg, ispE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SspF context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: switched on during stage III of sporulation PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Stephens et al. (1984) A promoter whose utilization is temporally regulated during sporulation in Bacillus subtilis. J. Mol. Biol. 176: 333-348. PubMed