Difference between revisions of "OppB"

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(Biological materials)
(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
 
** GP2063 (''[[oppB]]''::''spc''), available in [[Jörg Stülke]]'s lab
 
** GP2063 (''[[oppB]]''::''spc''), available in [[Jörg Stülke]]'s lab
 +
** GP2097 (D(''[[oppA]]-[[oppB]]-[[oppC]]-[[oppD]]-[[oppF]]'')::''aphA3''), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:43, 20 July 2015

Gene name oppB
Synonyms spo0KB
Essential no
Product oligopeptide ABC transporter (permease)
Function initiation of sporulation, competence development
Gene expression levels in SubtiExpress: oppB
Interactions involving this protein in SubtInteract: OppB
Metabolic function and regulation of this protein in SubtiPathways:
oppB
MW, pI 33 kDa, 9.863
Gene length, protein length 933 bp, 311 aa
Immediate neighbours oppA, oppC
Sequences Protein DNA DNA_with_flanks
Genetic context
OppB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OppB expression.png















Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, genetic competence, phosphorelay, membrane proteins

This gene is a member of the following regulons

CodY regulon, ScoC regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU11440

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • required for the uptake of quorum sensing peptides such as PhrH PubMed
  • Protein family: ABC transmembrane type-1 domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during exponential growth (ScoC) PubMed
  • Regulatory mechanism:
    • TnrA: transcription activation PubMed
    • ScoC: transcription repression PubMed
    • CodY: transcription repression, no derepression occurs, however, in a codY mutant, due to increased repression by ScoC PubMed
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 835 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References