BcaP

• Description: branched-chain amino acid transporter, major transporter for isoleucine and valine, may also be involved in threonine transport
  
  

Gene name	bcaP
Synonyms	yhdG
Essential	no
Product	branched-chain amino acid transporter
Function	biosynthesis/acquisition of branched-chain amino acids
Gene expression levels in SubtiExpress: bcaP
Metabolic function and regulation of this protein in SubtiPathways: BcaP
MW, pI	49 kDa, 9.735
Gene length, protein length	1395 bp, 465 aa
Immediate neighbours	yhdF, yhdH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU09460

Phenotypes of a mutant

• no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed
• resistant to growth inhibition by high concentrations of threonine PubMed

Database entries

• BsubCyc: BSU09460

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • high affinity uptake of isoleucine and valine PubMed

• Protein family:
  • amino acid, polyamine, organocation (APC) superfamily of transporters, TC 2.A.3

• Paralogous protein(s): YfnA

Extended information on the protein

• Kinetic information:
  • for Ile: Km ≈ 4.1 μM; Vmax at 26°C was ~15 nmol/min/ mg protein PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU09460

• Structure:

• UniProt: O07576

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: bcaP PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: BKE09460 (bcaP::erm, available in the BGSC and in Fabian Commichau's and Jörg Stülke's labs)

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: