ComGA

• Description: late competence protein, traffic ATPase, binds and transports transforming DNA
  
  

Gene name	comGA
Synonyms
Essential	no
Product	traffic ATPase
Function	genetic competence
Gene expression levels in SubtiExpress: comGA
Interactions involving this protein in SubtInteract: ComGA
Metabolic function and regulation of this protein in SubtiPathways: ComGA
MW, pI	40 kDa, 8.893
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	comGB, corA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU24730

Database entries

• BsubCyc: BSU24730

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds and transports transforming DNA

• Protein family: GSP E family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGA-ComGB PubMed
  • ComGA-ComFA PubMed
  • ComEC-ComGA PubMed
  • Maf-ComGA PubMed

• Localization:
  • peripheral membrane protein PubMed
  • localizes to cell poles in competent cells PubMed
  • localizes to one cell pole PubMed

Database entries

• BsubCyc: BSU24730

• Structure:

• UniProt: P25953

• KEGG entry: [3]

• E.C. number:

Additional information

• ComGA stability depends on Maf PubMed

Expression and regulation

• Operon: comGA-comGB-comGC-comGD-comGE-comGF-comGG-yqzE PubMed

• Expression browser: comGA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:
  • GP1640 trpC2 ymdB::spc comG-gfp (spc), available in Jörg Stülke's lab
  • GP1643 comG-gfp (spc) in the NCIB3610 background, available in Jörg Stülke's lab

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)