Difference between revisions of "HemQ"
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* '''Expression vector:''' | * '''Expression vector:''' | ||
− | ** | + | **pGP2644: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Jörg Stülke]]'s lab |
* '''lacZ fusion:''' | * '''lacZ fusion:''' |
Revision as of 11:36, 1 April 2015
- Description: heme-binding protein, essential for heme biosynthesis
Gene name | hemQ |
Synonyms | ipa-87r, ywfI |
Essential | no |
Product | heme-binding protein |
Function | biosynthesis of heme |
Gene expression levels in SubtiExpress: hemQ | |
Metabolic function and regulation of this protein in SubtiPathways: HemQ | |
MW, pI | 29 kDa, 4.972 |
Gene length, protein length | 762 bp, 254 aa |
Immediate neighbours | pta, bacG |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biosynthesis of cofactors, phosphoproteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37670
Phenotypes of a mutant
defect in heme biosynthesis
Database entries
- BsubCyc: BSU37670
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: UPF0447 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on Ser-41 PubMed
- Cofactor(s): heme
- Effectors of protein activity:
Database entries
- BsubCyc: BSU37670
- Structure: 1T0T (Geobacillus stearothermophilus)
- UniProt: P39645
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: hemQ PubMed
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- pGP2644: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References