NadE

• Description: NH3-dependent NAD+ synthetase
  
  

Gene name	nadE
Synonyms	outB, tscBGH, gsp-81
Essential	yes PubMed
Product	NH3-dependent NAD+ synthetase
Function	NAD biosynthesis
Gene expression levels in SubtiExpress: nadE
Metabolic function and regulation of this protein in SubtiPathways: NadE
MW, pI	30 kDa, 4.888
Gene length, protein length	816 bp, 272 aa
Immediate neighbours	ycgI, tmrB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, general stress proteins (controlled by SigB), essential genes

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

• Locus tag: BSU03130

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU03130

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + deamido-NAD⁺ + NH₃ = AMP + diphosphate + NAD⁺ (according to Swiss-Prot)

• Protein family: NAD synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU03130

• Structure: 1KQP

• UniProt: P08164

• KEGG entry: [3]

• E.C. number: 6.3.1.5

Expression and regulation

• Operon: nadE PubMed

• Expression browser: nadE PubMed

• Sigma factor: SigA, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • induced by antibiotics interacting with the cytoplasmic membrane PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 684 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 6179 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Michaela Wenzel, Bastian Kohl, Daniela Münch, Nadja Raatschen, H Bauke Albada, Leendert Hamoen, Nils Metzler-Nolte, Hans-Georg Sahl, Julia E Bandow
Proteomic response of Bacillus subtilis to lantibiotics reflects differences in interaction with the cytoplasmic membrane.
Antimicrob Agents Chemother: 2012, 56(11);5749-57
[PubMed:22926563] [WorldCat.org] [DOI] (I p)

James A Mobley, Anton Poliakov
Detection of early unfolding events in a dimeric protein by amide proton exchange and native electrospray mass spectrometry.
Protein Sci: 2009, 18(8);1620-7
[PubMed:19554628] [WorldCat.org] [DOI] (I p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

H Antelmann, R Schmid, M Hecker
The NAD synthetase NadE (OutB) of Bacillus subtilis is a sigma B-dependent general stress protein.
FEMS Microbiol Lett: 1997, 153(2);405-9
[PubMed:9271869] [WorldCat.org] [DOI] (P p)

T Caramori, S Calogero, A M Albertini, A Galizzi
Functional analysis of the outB gene of Bacillus subtilis.
J Gen Microbiol: 1993, 139(1);31-7
[PubMed:8450306] [WorldCat.org] [DOI] (P p)