NadC

• Description: nicotinate-nucleotide diphosphorylase (carboxylating)
  
  

Gene name	nadC
Synonyms	yrxB
Essential	no
Product	nicotinate-nucleotide diphosphorylase (carboxylating)
Function	NAD biosynthesis
Gene expression levels in SubtiExpress: nadC
Metabolic function and regulation of this protein in SubtiPathways: NadC
MW, pI	31 kDa, 5.18
Gene length, protein length	867 bp, 289 aa
Immediate neighbours	nadA, nadB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

• Locus tag: BSU27860

Database entries

• BsubCyc: BSU27860

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Nicotinate D-ribonucleotide + diphosphate + CO₂ = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: nadC/modD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU27860

• Structure:

• UniProt: P39666

• KEGG entry: [3]

• E.C. number: 2.4.2.19

Expression and regulation

• Operon: nadB-nadC-nadA PubMed

• Expression browser: nadC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of nicotinic acid (NadR) PubMed

• Regulatory mechanism:
  • NadR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1504 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 4421 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.
J Bacteriol: 1993, 175(5);1423-32
[PubMed:8444804] [WorldCat.org] [DOI] (P p)