Difference between revisions of "Obg"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 406 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 981 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:38, 17 April 2014

  • Description: GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit

Gene name obg
Synonyms
Essential Yes
Product GTP-binding protein
Function ribosome assembly, may be required to stimulate activity

of the phosphorelay that activates Spo0A

Gene expression levels in SubtiExpress: obg

of the phosphorelay that activates Spo0A

Interactions involving this protein in SubtInteract: Obg
MW, pI 47 kDa, 4.859
Gene length, protein length 1284 bp, 428 aa
Immediate neighbours pheB, spo0B
Sequences Protein DNA DNA_with_flanks
Genetic context
Obg context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Obg expression.png
























Categories containing this gene/protein

translation, phosphorelay, essential genes, GTP-binding proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27920

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: GTP1/OBG family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:

Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.

  • Modification:
  • Cofactor(s):

GDP, GTP and probably ppGpp

  • Effectors of protein activity:

Interaction with Rsb proteins.

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 406 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 981 PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP835, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP843, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications