NudF

• Description: involved in isopentenol (isoprenoid) biosynthesis
  
  

Gene name	nudF
Synonyms	yqkG
Essential	no
Product	unknown
Function	isoprenoid biosynthesis
Gene expression levels in SubtiExpress: nudF
MW, pI	20 kDa, 4.586
Gene length, protein length	555 bp, 185 aa
Immediate neighbours	yqxK, mciZ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids

The gene

Basic information

• Locus tag: BSU23610

Database entries

• BsubCyc: BSU23610

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ADP-ribose + H₂O = AMP + D-ribose 5-phosphate (according to Swiss-Prot)

• Protein family: Nudix hydrolase, NudF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23610

• Structure:

• UniProt: P54570

• KEGG entry: [2]

• E.C. number: 3.6.1.13

Expression and regulation

• Operon:

• Expression browser: nudF PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 261 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 469 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sydnor T Withers, Shayin S Gottlieb, Bonny Lieu, Jack D Newman, Jay D Keasling
Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity.
Appl Environ Microbiol: 2007, 73(19);6277-83
[PubMed:17693564] [WorldCat.org] [DOI] (P p)

C A Dunn, S F O'Handley, D N Frick, M J Bessman
Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance.
J Biol Chem: 1999, 274(45);32318-24
[PubMed:10542272] [WorldCat.org] [DOI] (P p)