Difference between revisions of "NudF"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 261 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 469 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:32, 17 April 2014

  • Description: involved in isopentenol (isoprenoid) biosynthesis

Gene name nudF
Synonyms yqkG
Essential no
Product unknown
Function isoprenoid biosynthesis
Gene expression levels in SubtiExpress: nudF
MW, pI 20 kDa, 4.586
Gene length, protein length 555 bp, 185 aa
Immediate neighbours yqxK, mciZ
Sequences Protein DNA DNA_with_flanks
Genetic context
NudF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NudF expression.png
























Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23610

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ADP-ribose + H2O = AMP + D-ribose 5-phosphate (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 261 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 469 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Sydnor T Withers, Shayin S Gottlieb, Bonny Lieu, Jack D Newman, Jay D Keasling
Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity.
Appl Environ Microbiol: 2007, 73(19);6277-83
[PubMed:17693564] [WorldCat.org] [DOI] (P p)

C A Dunn, S F O'Handley, D N Frick, M J Bessman
Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance.
J Biol Chem: 1999, 274(45);32318-24
[PubMed:10542272] [WorldCat.org] [DOI] (P p)