YhxA

• Description: similar to adenosylmethionine-8-amino-7-oxononanoate aminotransferase
  
  

Gene name	yhxA
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yhxA
MW, pI	49 kDa, 5.644
Gene length, protein length	1350 bp, 450 aa
Immediate neighbours	yhzG, glpP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

The gene

Basic information

• Locus tag: BSU09260

Database entries

• BsubCyc: BSU09260

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: class-III pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU09260

• Structure:

• UniProt: P33189

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: yhxA-glpP PubMed

• Expression browser: yhxA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • half-life of the mRNA: 3.7 min PubMed
  • the mRNA is stabilized in rppH and rnjA mutants PubMed
  • the mRNA is subject to degradation by RNase J1 PubMed
  • subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 538 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 599 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jamie Richards, Quansheng Liu, Olivier Pellegrini, Helena Celesnik, Shiyi Yao, David H Bechhofer, Ciarán Condon, Joel G Belasco
An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.
Mol Cell: 2011, 43(6);940-9
[PubMed:21925382] [WorldCat.org] [DOI] (I p)

Ulf Gerth, Holger Kock, Ilja Kusters, Stephan Michalik, Robert L Switzer, Michael Hecker
Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis.
J Bacteriol: 2008, 190(1);321-31
[PubMed:17981983] [WorldCat.org] [DOI] (I p)

C Holmberg, L Beijer, B Rutberg, L Rutberg
Glycerol catabolism in Bacillus subtilis: nucleotide sequence of the genes encoding glycerol kinase (glpK) and glycerol-3-phosphate dehydrogenase (glpD).
J Gen Microbiol: 1990, 136(12);2367-75
[PubMed:2127799] [WorldCat.org] [DOI] (P p)