yhxA

168

similar to adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Locus: BSU_09260

Molecular weight: 49.71 kDa

Isoelectric point: 5.64

Protein length: 450 aa

Gene length: 1350 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhxA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0161 (Galperin et al., 2021)

Gene

Coordinates: 1,000,364 → 1,001,716

The protein

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 3N5M (PDB) (from Bacillus Anthracis, 64% identity)
• P33189 (AlphaFold)

Paralogous protein(s)

• ArgD, RocD
• YodT, (34,9%)
• BioA, (33,6%)
• GabT, (32.8%)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: yhxA-glpP

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • half-life of the mRNA: 3.7 min PubMed
  • half-life of the mRNA: 3.7 min PubMed

Biological materials

Mutant

• MGNA-B475 (yhxA::erm), available at the NBRP B. subtilis, Japan
• BKE09260 (ΔyhxA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCATTAGCGTTCCCTA,  downstream forward: _UP4_TAATATAAAAGTGAATTATT
• BKK09260 (ΔyhxA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCATTAGCGTTCCCTA,  downstream forward: _UP4_TAATATAAAAGTGAATTATT

References

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