PurS

• Description: phosphoribosylformylglycinamidine synthase
  
  

Gene name	purS
Synonyms	yexA
Essential	no
Product	phosphoribosylformylglycinamidine synthase
Function	purine biosynthesis
Gene expression levels in SubtiExpress: purS
Interactions involving this protein in SubtInteract: PurS
Metabolic function and regulation of this protein in SubtiPathways: purS
MW, pI	9 kDa, 4.594
Gene length, protein length	252 bp, 84 aa
Immediate neighbours	purC, purQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, most abundant proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

• Locus tag: BSU06460

Database entries

• BsubCyc: BSU06460

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0062 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • PurS-PurS PubMed
  • PurS-PurQ-PurL PubMed

• Localization:

Database entries

• BsubCyc: BSU06460

• Structure: 1T4A

• UniProt: P12049

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD PubMed

• Expression browser: purS PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of purine nucleotides (PurR) PubMed
  • repressed in the presence of adenine or adenosine(PurR) PubMed
  • repressed in the presence of guanine(G-box) PubMed

• Regulatory mechanism:
  • [PurR]]: transcription repression PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • G-box: transcription termination/ antitermination (riboswitch) PubMed1 PubMed2

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3192 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 6989 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: