Difference between revisions of "Hag"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[motility and chemotaxis]]}} | + | {{SubtiWiki category|[[motility and chemotaxis]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
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| − | |||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
** the ''[[hag]]'' gene is strongly overexpressed in a ''[[ymdB]]'' mutant (loss of bistable gene expression) {{PubMed|21856853}} | ** the ''[[hag]]'' gene is strongly overexpressed in a ''[[ymdB]]'' mutant (loss of bistable gene expression) {{PubMed|21856853}} | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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==Original Publications== | ==Original Publications== | ||
| − | <pubmed> 23144244 21602220 21856853 2498284,9648743,9096206,7708689,19202088, 18763711, 21895793 10809682 19118355 17555441 20534509 </pubmed> | + | <pubmed> 23144244 21602220 21856853 2498284,9648743,9096206,7708689,19202088, 18763711, 21895793 10809682 19118355 17555441 20534509 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:54, 5 March 2014
- Description: flagellin protein, about 20,000 subunits make up one flagellum
| Gene name | hag |
| Synonyms | |
| Essential | no |
| Product | flagellin protein |
| Function | motility and chemotaxis |
| Gene expression levels in SubtiExpress: hag | |
| Interactions involving this protein in SubtInteract: Hag | |
| MW, pI | 32 kDa, 4.782 |
| Gene length, protein length | 912 bp, 304 aa |
| Immediate neighbours | yvyC, csrA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
motility and chemotaxis, most abundant proteins
This gene is a member of the following regulons
CodY regulon, CsrA regulon, ScoC regulon, SigD regulon
The gene
Basic information
- Locus tag: BSU35360
Phenotypes of a mutant
No swarming motility on B medium. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: bacterial flagellin family (according to Swiss-Prot)
- Paralogous protein(s): YvzB (C-terminal domain of Hag)
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure: 3A5X (from Salmonella typhimurium, 42% identity)
- UniProt: P02968
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- the hag gene is strongly overexpressed in a ymdB mutant (loss of bistable gene expression) PubMed
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant: GP901 (aphA3), GP902 (tet) PubMed, both available in Jörg Stülke's lab
- Expression vector:
- expression in B. subtilis, in pBQ200: pGP1089, available in Jörg Stülke's lab
- lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7 PubMed, all available in Jörg Stülke's lab
- GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Jörg Stülke's lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Publications
