PlsX

• Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division
  
  

Gene name	plsX
Synonyms	ylpD
Essential	yes PubMed
Product	acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function	biosynthesis of phospholipids
Gene expression levels in SubtiExpress: plsX
Interactions involving this protein in SubtInteract: PlsX
Metabolic function and regulation of this protein in SubtiPathways: plsX
MW, pI	35 kDa, 5.492
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	fapR, fabD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, biosynthesis of lipids, essential genes

This gene is a member of the following regulons

ComA regulon, FapR regulon

The gene

Basic information

• Locus tag: BSU15890

Phenotypes of a mutant

• essential PubMed
• depletion results in sporulation deficiency (via SpoIIR) PubMed
• a ts mutant forms an abberant Z-ring and has a cell division defect PubMed

Database entries

• BsubCyc: BSU15890

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

• Protein family: plsX family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • PlsX-PlsY PubMed
  • PlsX-FtsA PubMed

• Localization:
  • localizes to the cell division sites, colocalizes with the Z-ring PubMed

Database entries

• BsubCyc: BSU15890

• Structure: 1VI1

• UniProt: P71018

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fapR-plsX-fabD-fabG-acpA PubMed

• Expression browser: plsX PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
  • expressed at high cell density (ComA) PubMed
  • induced upon fatty acid biosynthesis inhibition PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • FapR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed

• Antibody: