Difference between revisions of "RocF"

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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:''' phosphorylated on Ser-68 {{PubMed|20509597}}
 
* '''Modification:''' phosphorylated on Ser-68 {{PubMed|20509597}}
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EF5 2EF5] (from ''Thermus thermophilus'', 46% identity, 63% similarity)
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* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2CEV 2CEV] (from ''Bacillus caldevelox'', 71% identity) {{PubMed|10196128}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39138 P39138]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39138 P39138]
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=References=
 
=References=
  
<pubmed>12618455, 20509597,14651647,12618455,7540694, 23625224 </pubmed>
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<pubmed>12618455, 20509597,14651647,12618455,7540694, 23625224 10196128 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:09, 27 February 2014

  • Description: arginase

Gene name rocF
Synonyms
Essential no
Product arginase
Function arginine utilization
Gene expression levels in SubtiExpress: rocF
Metabolic function and regulation of this protein in SubtiPathways:
rocF
MW, pI 32 kDa, 4.932
Gene length, protein length 888 bp, 296 aa
Immediate neighbours phrG, rocE
Sequences Protein DNA DNA_with_flanks
Genetic context
RocF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RocF expression.png















Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU40320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-arginine + H2O = L-ornithine + urea (according to Swiss-Prot)
  • Protein family: arginase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification: phosphorylated on Ser-68 PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 2CEV (from Bacillus caldevelox, 71% identity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by arginine (RocR, AhrC) PubMed
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant: GP655, aphA3, available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References