Difference between revisions of "Tpi"
| Line 44: | Line 44: | ||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
{{SubtiWiki category|[[carbon core metabolism]]}}, | {{SubtiWiki category|[[carbon core metabolism]]}}, | ||
| − | {{SubtiWiki category|[[phosphoproteins]]}} | + | {{SubtiWiki category|[[phosphoproteins]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' phosphorylation on Ser-213 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed] | * '''Modification:''' phosphorylation on Ser-213 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed] | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' inhibited by 2-phosphoglycolate (in ''B. stearothermophilus'') [http://www.ncbi.nlm.nih.gov/sites/entrez/8580851 PubMed] | * '''Effectors of protein activity:''' inhibited by 2-phosphoglycolate (in ''B. stearothermophilus'') [http://www.ncbi.nlm.nih.gov/sites/entrez/8580851 PubMed] | ||
| Line 126: | Line 127: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
| Line 150: | Line 152: | ||
=References= | =References= | ||
| − | <pubmed>12850135 11489127 17505547 8021172 17218307 8580851 19193632 21821766 23420519</pubmed> | + | <pubmed>12850135 11489127 17505547 8021172 17218307 8580851 19193632 21821766 23420519 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:37, 5 March 2014
- Description: triose phosphate isomerase, glycolytic/ gluconeogenic enzyme
| Gene name | tpi |
| Synonyms | tpiA |
| Essential | no |
| Product | triosephosphate isomerase |
| Function | enzyme in glycolysis/ gluconeogenesis |
| Gene expression levels in SubtiExpress: tpi | |
| Interactions involving this protein in SubtInteract: Tpi | |
| Metabolic function and regulation of this protein in SubtiPathways: tpi | |
| MW, pI | 26,9 kDa, 4.79 |
| Gene length, protein length | 759 bp, 253 amino acids |
| Immediate neighbours | pgm, pgk |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
carbon core metabolism, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33920
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
- A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
- Protein family: triosephosphate isomerase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification: phosphorylation on Ser-213 PubMed
- Effectors of protein activity: inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed
- Localization:
- cytoplasm PubMed
Database entries
- Structure: 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI
- UniProt: P27876
- KEGG entry: [3]
- E.C. number: 5.3.1.1
Additional information
- extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia
Expression and regulation
- Regulation:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant: GP700 (cat), available in Jörg Stülke's lab, PubMed
- Expression vector:
- pGP394 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Jörg Stülke's lab
- pGP1511 (expression in B. subtilis, in pBQ200), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
