SpoIIAB

• Description: anti-SigF/ protein serine kinase
  
  

Gene name	spoIIAB
Synonyms
Essential	no
Product	anti-SigF/ protein serine kinase
Function	control of SigF activity septation; phosphorylation and inactivation of SpoIIAA
Gene expression levels in SubtiExpress: spoIIAB
Interactions involving this protein in SubtInteract: SpoIIAB
Metabolic function and regulation of this protein in SubtiPathways: spoIIAB
MW, pI	16 kDa, 4.383
Gene length, protein length	438 bp, 146 aa
Immediate neighbours	sigF, spoIIAA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, sigma factors and their control, sporulation proteins

This gene is a member of the following regulons

AbrB regulon, SigF regulon, SigG regulon, SigH regulon, SinR regulon, Spo0A regulon

The gene

Basic information

• Locus tag: BSU23460

Database entries

• BsubCyc: BSU23460

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)

• Protein family: anti-sigma-factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIIE-SpoIIAB, RsbV-SpoIIAB PubMed
  • SpoIIAB-SigG PubMed
  • SpoIIAB-SigF, SpoIIAB-SpoIIAA PubMed

• Localization:

Database entries

• BsubCyc: BSU23460

• Structure: 1L0O (complex with SigF, Geobacillus stearothermophilus), 1TIL (complex with SpoIIAA, Geobacillus stearothermophilus)

• UniProt: P10728

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • dacF-spoIIAA-spoIIAB-sigF
  • spoIIAA-spoIIAB-sigF

• Expression browser: spoIIAB PubMed

• Sigma factor:
  • dacF: SigF, SigG
  • spoIIAA: SigH

• Regulation:
  • dacF: expressed during sporulation
  • spoIIAA: expressed early during sporulation
  • expressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism: spoIIAA: activated by Spo0A and repressed by SinR
  • Spo0A: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Charles Moran, Emory University, NC, USA homepage

References

Modeling of SigF activation

Georgios Fengos, Dagmar Iber
Prediction stability in a data-based, mechanistic model of σF regulation during sporulation in Bacillus subtilis.
Sci Rep: 2013, 3;2755
[PubMed:24067622] [WorldCat.org] [DOI] (I e)

Dagmar Iber
Inferring Biological Mechanisms by Data-Based Mathematical Modelling: Compartment-Specific Gene Activation during Sporulation in Bacillus subtilis as a Test Case.
Adv Bioinformatics: 2011, 2011;124062
[PubMed:22312331] [WorldCat.org] [DOI] (I p)

Jin Hwan Do, Masao Nagasaki, Satoru Miyano
The systems approach to the prespore-specific activation of sigma factor SigF in Bacillus subtilis.
Biosystems: 2010, 100(3);178-84
[PubMed:20298743] [WorldCat.org] [DOI] (I p)