Difference between revisions of "PanB"
| Line 58: | Line 58: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22430&redirect=T BSU22430] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
| Line 96: | Line 97: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22430&redirect=T BSU22430] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3EZ4 3EZ4] (from ''Burkholderia pseudomallei'', 44% identity, 64% similarity) | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3EZ4 3EZ4] (from ''Burkholderia pseudomallei'', 44% identity, 64% similarity) | ||
Revision as of 14:02, 2 April 2014
- Description: 3-methyl-2-oxobutanoate hydroxymethyltransferase
| Gene name | panB |
| Synonyms | |
| Essential | no |
| Product | 3-methyl-2-oxobutanoate hydroxymethyltransferase |
| Function | biosynthesis of coenzyme A |
| Gene expression levels in SubtiExpress: panB | |
| Metabolic function and regulation of this protein in SubtiPathways: panB | |
| MW, pI | 29 kDa, 5.282 |
| Gene length, protein length | 831 bp, 277 aa |
| Immediate neighbours | panC, birA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22430
Phenotypes of a mutant
Database entries
- BsubCyc: BSU22430
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU22430
- Structure: 3EZ4 (from Burkholderia pseudomallei, 44% identity, 64% similarity)
- UniProt: P52996
- KEGG entry: [2]
- E.C. number: 2.1.2.11
Additional information
Expression and regulation
- Operon:
- Regulation:
- induced upon fatty acid biosynthesis inhibition PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: PA126 (panB::cat), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Michaela Wenzel, Malay Patra, Dirk Albrecht, David Y-K Chen, K C Nicolaou, Nils Metzler-Nolte, Julia E Bandow
Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies.
Antimicrob Agents Chemother: 2011, 55(6);2590-6
[PubMed:21383089]
[WorldCat.org]
[DOI]
(I p)
