ThiT

• Description: S protein of thiamine ECF transporter
  
  

Gene name	thiT
Synonyms	yuaJ
Essential	no
Product	S protein of thiamine ECF transporter
Function	thiamine uptake
Gene expression levels in SubtiExpress: thiT
Interactions involving this protein in SubtInteract: ThiT
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	20 kDa, 10.237
Gene length, protein length	576 bp, 192 aa
Immediate neighbours	rrnB-16S, yuaI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ECF transporter, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU30990

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: proton-coupled thiamine transporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YbaF-ThiT PubMed

• Localization:
  • membrane PubMed

Database entries

• Structure: 3RLB (from Lactococcus lactis, 32% identity) PubMed

• UniProt: O32074

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: thiT PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Guus B Erkens, Maria Majsnerowska, Josy ter Beek, Dirk Jan Slotboom
Energy coupling factor-type ABC transporters for vitamin uptake in prokaryotes.
Biochemistry: 2012, 51(22);4390-6
[PubMed:22574898] [WorldCat.org] [DOI] (I p)

Thomas Eitinger, Dmitry A Rodionov, Mathias Grote, Erwin Schneider
Canonical and ECF-type ATP-binding cassette importers in prokaryotes: diversity in modular organization and cellular functions.
FEMS Microbiol Rev: 2011, 35(1);3-67
[PubMed:20497229] [WorldCat.org] [DOI] (I p)

Original publications

Maria Majsnerowska, Inga Hänelt, Dorith Wunnicke, Lars V Schäfer, Heinz-Jürgen Steinhoff, Dirk Jan Slotboom
Substrate-induced conformational changes in the S-component ThiT from an energy coupling factor transporter.
Structure: 2013, 21(5);861-7
[PubMed:23602660] [WorldCat.org] [DOI] (I p)

Guus B Erkens, Ronnie P-A Berntsson, Faizah Fulyani, Maria Majsnerowska, Andreja Vujičić-Žagar, Josy Ter Beek, Bert Poolman, Dirk Jan Slotboom
The structural basis of modularity in ECF-type ABC transporters.
Nat Struct Mol Biol: 2011, 18(7);755-60
[PubMed:21706007] [WorldCat.org] [DOI] (I e)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)