YaaH

• Description: general stress protein, survival of ethanol stress, SafA-dependent protein of the inner spore coat, spore cortex lytic protein
  
  

Gene name	yaaH
Synonyms	sleL
Essential	no
Product	N-acetylglucosaminidase
Function	survival of ethanol stress, protection of the spore
Gene expression levels in SubtiExpress: yaaH
MW, pI	48 kDa, 5.618
Gene length, protein length	1281 bp, 427 aa
Immediate neighbours	dgk, yaaI
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon, SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU00160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • contains two N-acetylglucosamine-polymer-binding LysM domains at the N-terminus PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • inner spore coat, localization depends on SafA PubMed

Database entries

• Structure:

• UniProt: P37531

• KEGG entry: [3]

• E.C. number:

Additional information

required for survival of ethanol stress PubMed

Expression and regulation

• Operon: yaaH PubMed

• Expression browser: yaaH PubMed

• Sigma factor: SigE PubMed, SigB PubMed

• Regulation:
  • induced by stress (SigB) PubMed
  • expressed during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Girbe Buist, Anton Steen, Jan Kok, Oscar P Kuipers
LysM, a widely distributed protein motif for binding to (peptido)glycans.
Mol Microbiol: 2008, 68(4);838-47
[PubMed:18430080] [WorldCat.org] [DOI] (I p)

Original publications

Emily A Lambert, Nora Sherry, David L Popham
In vitro and in vivo analyses of the Bacillus anthracis spore cortex lytic protein SleL.
Microbiology (Reading): 2012, 158(Pt 5);1359-1368
[PubMed:22343356] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Emily A Lambert, David L Popham
The Bacillus anthracis SleL (YaaH) protein is an N-acetylglucosaminidase involved in spore cortex depolymerization.
J Bacteriol: 2008, 190(23);7601-7
[PubMed:18835992] [WorldCat.org] [DOI] (I p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

A Aronson
Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.
Cell Mol Life Sci: 2002, 59(3);417-25
[PubMed:11964120] [WorldCat.org] [DOI] (P p)

T Kodama, H Takamatsu, K Asai, K Kobayashi, N Ogasawara, K Watabe
The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.
J Bacteriol: 1999, 181(15);4584-91
[PubMed:10419957] [WorldCat.org] [DOI] (P p)