CotD

• Description: spore coat protein (inner)
  
  

Gene name	cotD
Synonyms
Essential	no
Product	spore coat protein (inner)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotD
MW, pI	8 kDa, 7.373
Gene length, protein length	225 bp, 75 aa
Immediate neighbours	ypsA, yprB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU22200

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nhaC Na(+)/H(+) antiporter family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • inner spore coat PubMed, localization depends on SafA PubMed

Database entries

• Structure:

• UniProt: P07791

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotD (according to DBTBS)

• Expression browser: cotD PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, SpoIIID, GerE) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed
  • GerE: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S104 ( cotD::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)