Difference between revisions of "ThiT"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3RLB 3RLB] (from ''Lactococcus lactis'', 32% identity) {{PubMed|21706007}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32074 O32074] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O32074 O32074] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thiT_3179306_3179884_1 thiT] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thiT_3179306_3179884_1 thiT] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed>16291685,,18763711, 12376536</pubmed> | + | <pubmed>16291685, 21706007,18763711, 12376536</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:25, 12 July 2013
- Description: thiamine permease
| Gene name | thiT |
| Synonyms | yuaJ |
| Essential | no |
| Product | thiamine permease |
| Function | thiamine uptake |
| Gene expression levels in SubtiExpress: thiT | |
| Metabolic function and regulation of this protein in SubtiPathways: Thiamin | |
| MW, pI | 20 kDa, 10.237 |
| Gene length, protein length | 576 bp, 192 aa |
| Immediate neighbours | rrnB-16S, yuaI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
transporters/ other, biosynthesis of cofactors, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU30990
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: proton-coupled thiamine transporter family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- membrane associated PubMed
Database entries
- UniProt: O32074
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Guus B Erkens, Ronnie P-A Berntsson, Faizah Fulyani, Maria Majsnerowska, Andreja Vujičić-Žagar, Josy Ter Beek, Bert Poolman, Dirk Jan Slotboom
The structural basis of modularity in ECF-type ABC transporters.
Nat Struct Mol Biol: 2011, 18(7);755-60
[PubMed:21706007]
[WorldCat.org]
[DOI]
(I e)
Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711]
[WorldCat.org]
[DOI]
(I p)
Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685]
[WorldCat.org]
[DOI]
(P p)
Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536]
[WorldCat.org]
[DOI]
(P p)
