NadB

• Description: L-aspartate oxidase
  
  

Gene name	nadB
Synonyms
Essential	no
Product	L-aspartate oxidase
Function	NAD biosynthesis
Gene expression levels in SubtiExpress: nadB
MW, pI	58 kDa, 6.445
Gene length, protein length	1593 bp, 531 aa
Immediate neighbours	nadC, nifS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

• Locus tag: BSU27870

Database entries

• BsubCyc: BSU27870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-aspartate + O₂ = iminosuccinate + H₂O₂ (according to Swiss-Prot)

• Protein family: NadB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-104 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27870

• Structure:

• UniProt: P38032

• KEGG entry: [3]

• E.C. number: 1.4.3.16

Expression and regulation

• Operon: nadB-nadC-nadA PubMed

• Expression browser: nadB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of nicotinic acid (NadR) PubMed

• Regulatory mechanism:
  • NadR: transcription repression PubMed

• Additional information:
  • An antisense RNA is predicted for nadB PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Alessandra Albertini, University of Pavia, Italy homepage