Difference between revisions of "SpoIIIC"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU26390 spoIIIC]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU26390 spoIIIC]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIIIC SpoIIIC]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=SpoIIIC SpoIIIC]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.608   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 9.608   

Revision as of 08:56, 12 November 2013

Gene name spoIIIC
Synonyms sigK, spoIVD, spoIVE
Essential no
Product RNA polymerase sporulation-specific sigma factor
(SigK) (C-terminal half)
Function late mother cell-specific gene expression
Gene expression levels in SubtiExpress: spoIIIC
Interactions involving this protein in SubtInteract: SpoIIIC
MW, pI 16 kDa, 9.608
Gene length, protein length 414 bp, 138 aa
Immediate neighbours yqaB, yrkS
Gene sequence (+200bp) Protein sequence
Genetic context
SpoIIIC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIIC expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

GerE regulon, SigE regulon, SigK regulon, SpoIIID regulon

The SigK regulon

The gene

Basic information

  • Locus tag: BSU26390

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • SigK is activated by processing of membrane-bound pro-SigK by the SpoIVFB metalloprotease PubMed
    • spoIVCB and spoIIIC form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

The SigK regulon

Jessica M Silvaggi, John B Perkins, Richard Losick
Genes for small, noncoding RNAs under sporulation control in Bacillus subtilis.
J Bacteriol: 2006, 188(2);532-41
[PubMed:16385044] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

Other original publications

Ruanbao Zhou, Kangming Chen, Xianling Xiang, Liping Gu, Lee Kroos
Features of Pro-σK important for cleavage by SpoIVFB, an intramembrane metalloprotease.
J Bacteriol: 2013, 195(12);2793-806
[PubMed:23585539] [WorldCat.org] [DOI] (I p)

B Kunkel, R Losick, P Stragier
The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene.
Genes Dev: 1990, 4(4);525-35
[PubMed:2163341] [WorldCat.org] [DOI] (P p)

P Stragier, B Kunkel, L Kroos, R Losick
Chromosomal rearrangement generating a composite gene for a developmental transcription factor.
Science: 1989, 243(4890);507-12
[PubMed:2536191] [WorldCat.org] [DOI] (P p)

J Errington, S Rong, M S Rosenkrantz, A L Sonenshein
Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC.
J Bacteriol: 1988, 170(3);1162-7
[PubMed:3125151] [WorldCat.org] [DOI] (P p)

J Errington
Two separable functional domains in the sigma-subunit of RNA polymerase in Bacillus subtilis?
FEBS Lett: 1987, 224(2);257-60
[PubMed:3121385] [WorldCat.org] [DOI] (P p)

S M Turner, J Errington, J Mandelstam
Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons.
J Gen Microbiol: 1986, 132(11);2995-3003
[PubMed:3114422] [WorldCat.org] [DOI] (P p)