Difference between revisions of "IolE"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[iolF]]'', ''[[iolD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[iolF]]'', ''[[iolD]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39720 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39720 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39720 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39720 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39720 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39720 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:iolE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:iolE_context.gif]]

Revision as of 11:41, 14 May 2013

  • Description: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Gene name iolE
Synonyms yxdE
Essential no
Product 2-keto-myo-inositol dehydratase,
dehydration of 2-keto-myo-inositol (2nd reaction)
Function myo-inositol catabolism
Gene expression levels in SubtiExpress: iolE
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 33 kDa, 5.209
Gene length, protein length 891 bp, 297 aa
Immediate neighbours iolF, iolD
Sequences Protein DNA DNA_with_flanks
Genetic context
IolE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IolE expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, IolR regulon

The gene

Basic information

  • Locus tag: BSU39720

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H2O (according to Swiss-Prot)
  • Protein family: iolE/mocC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3CNY (from Lactobacillus plantarum wcfs1 mutant, 63% identity, 78% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-ichi Yoshida, Masanori Yamaguchi, Tetsuro Morinaga, Masaki Kinehara, Maya Ikeuchi, Hitoshi Ashida, Yasutaro Fujita
myo-Inositol catabolism in Bacillus subtilis.
J Biol Chem: 2008, 283(16);10415-24
[PubMed:18310071] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Masanori Yamaguchi, Hideki Ikeda, Kaoru Omae, Ken-Ichi Tsurusaki, Yasutaro Fujita
The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase.
Microbiology (Reading): 2004, 150(Pt 3);571-580
[PubMed:14993306] [WorldCat.org] [DOI] (P p)

K I Yoshida, T Shibayama, D Aoyama, Y Fujita
Interaction of a repressor and its binding sites for regulation of the Bacillus subtilis iol divergon.
J Mol Biol: 1999, 285(3);917-29
[PubMed:9887260] [WorldCat.org] [DOI] (P p)

K I Yoshida, D Aoyama, I Ishio, T Shibayama, Y Fujita
Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis.
J Bacteriol: 1997, 179(14);4591-8
[PubMed:9226270] [WorldCat.org] [DOI] (P p)