Difference between revisions of "Tpi"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pgm]]'', ''[[pgk]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pgm]]'', ''[[pgk]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33920 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33920 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33920 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33920 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:tpi_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:tpi_context.gif]]

Revision as of 11:17, 14 May 2013

  • Description: triose phosphate isomerase, glycolytic/ gluconeogenic enzyme

Gene name tpi
Synonyms tpiA
Essential no
Product triosephosphate isomerase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: tpi
Interactions involving this protein in SubtInteract: Tpi
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 26,9 kDa, 4.79
Gene length, protein length 759 bp, 253 amino acids
Immediate neighbours pgm, pgk
Sequences Protein DNA DNA_with_flanks
Genetic context
Tpi context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tpi expression.png
















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
  • Protein family: triosephosphate isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-213 PubMed
  • Cofactor(s):
  • Effectors of protein activity: inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed

Database entries

  • Structure: 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:

Biological materials

  • Mutant: GP700 (cat), available in Stülke lab
  • Expression vector:
    • pGP394 (N-terminal His-tag, in pWH844), available in Stülke lab
    • pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Stülke lab
    • pGP1511 (expression in B. subtilis, in pBQ200), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References