Difference between revisions of "PucD"

From SubtiWiki
Jump to: navigation, search
Line 24: Line 24:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucE]]'', ''[[pucC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucE]]'', ''[[pucC]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32480 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32480 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32480 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32480 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32480 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32480 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pucD_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pucD_context.gif]]

Revision as of 11:11, 14 May 2013

  • Description: xanthine dehydrogenase

Gene name pucD
Synonyms yurC
Essential no
Product xanthine dehydrogenase
Function purine utilization
Gene expression levels in SubtiExpress: pucD
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Purine catabolism, Nucleotides (regulation)
MW, pI 80 kDa, 5.754
Gene length, protein length 2235 bp, 745 aa
Immediate neighbours pucE, pucC
Sequences Protein DNA DNA_with_flanks
Genetic context
PucD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PucD expression.png




























Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon

The gene

Basic information

  • Locus tag: BSU32480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
  • Protein family: xanthine dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of purine nucleotides (effector: allantoin) (PucR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J Bacteriol: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)