Difference between revisions of "RibE"

From SubtiWiki
Jump to: navigation, search
Line 26: Line 26:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ribA]]'', ''[[ribD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ribA]]'', ''[[ribD]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23270 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23270 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ribE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ribE_context.gif]]

Revision as of 10:37, 14 May 2013

  • Description: riboflavin synthase (alpha subunit)

Gene name ribE
Synonyms ribB
Essential no
Product riboflavin synthase (alpha subunit)
Function riboflavin biosynthesis
Gene expression levels in SubtiExpress: ribE
Interactions involving this protein in SubtInteract: RibE
Metabolic function and regulation of this protein in SubtiPathways:
Riboflavin / FAD
MW, pI 23 kDa, 5.845
Gene length, protein length 645 bp, 215 aa
Immediate neighbours ribA, ribD
Sequences Protein DNA DNA_with_flanks
Genetic context
RibE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RibE expression.png















Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

FMN-box

The gene

Basic information

  • Locus tag: BSU23270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1I8D (from E. coli, 36% identity, 59% similarity) PubMed, , 1RVV (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed
  • Additional information:

Biological materials

  • Mutant: GP203 (erm), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References