Difference between revisions of "YvcJ"

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* '''Description:''' write here <br/><br/>
+
* '''Description:''' nucleotide-binding protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
+
|style="background:#ABCDEF;" align="center"| '''Product''' || nucleotide-binding P-loop-containing protein
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 6.702   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 6.702   
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
reduced competence
  
 
=== Database entries ===
 
=== Database entries ===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' binds and hydrolyzes nucleotides, required for the expression of late competence genes (''[[comK]], [[comS]]'')  [http://www.ncbi.nlm.nih.gov/sites/entrez/19074378 PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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# Galinier, A., Haiech, J., Kilhoffer, M.-C., Jaquinod, M., Stülke, J., Deutscher, J., & Martin-Verstraete, I. (1997) The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc. Natl. Acad. Sci. USA 94: 8439-8444. [http://www.ncbi.nlm.nih.gov/sites/entrez/9237995 PubMed]
 
# Galinier, A., Haiech, J., Kilhoffer, M.-C., Jaquinod, M., Stülke, J., Deutscher, J., & Martin-Verstraete, I. (1997) The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc. Natl. Acad. Sci. USA 94: 8439-8444. [http://www.ncbi.nlm.nih.gov/sites/entrez/9237995 PubMed]
 
# Görke et al. (2005) YvcK of ''Bacillus subtilis'' is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway. Microbiology 151: 3777-3791. [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]
 
# Görke et al. (2005) YvcK of ''Bacillus subtilis'' is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway. Microbiology 151: 3777-3791. [http://www.ncbi.nlm.nih.gov/sites/entrez/16272399 PubMed]
 +
#  Luciano: , E. Foulquier, J.-R. Fantino, A. Galinier, and F. Pompeo (2009) Characterization of YvcJ, a conserved P-loop-containing protein, and Its implication in competence in ''Bacillus subtilis''. J. Bacteriol. 191: 1556-1564-3791. [http://www.ncbi.nlm.nih.gov/sites/entrez/19074378 PubMed]

Revision as of 07:39, 18 February 2009

  • Description: nucleotide-binding protein

Gene name yvcJ
Synonyms
Essential no
Product nucleotide-binding P-loop-containing protein
Function required for the expression of late competence genes
MW, pI 33 kDa, 6.702
Gene length, protein length 885 bp, 295 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
YvcJ context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

reduced competence

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Galinier, A., Haiech, J., Kilhoffer, M.-C., Jaquinod, M., Stülke, J., Deutscher, J., & Martin-Verstraete, I. (1997) The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression. Proc. Natl. Acad. Sci. USA 94: 8439-8444. PubMed
  2. Görke et al. (2005) YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway. Microbiology 151: 3777-3791. PubMed
  3. Luciano: , E. Foulquier, J.-R. Fantino, A. Galinier, and F. Pompeo (2009) Characterization of YvcJ, a conserved P-loop-containing protein, and Its implication in competence in Bacillus subtilis. J. Bacteriol. 191: 1556-1564-3791. PubMed