Difference between revisions of "WapA"

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(The protein)
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* '''Catalyzed reaction/ biological activity:'''  
 
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** the C-terminal toxic domain has RNase activity (cleaves tRNAs) {{PubMed|23572593}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''

Revision as of 10:50, 19 April 2013

  • Description: cell wall-associated protein precursor, contact-dependent growth inhibition protein

Gene name wapA
Synonyms
Essential no
Product cell wall-associated protein precursor
Function intercellular competition
Gene expression levels in SubtiExpress: wapA
Interactions involving this protein in SubtInteract: WapA
MW, pI 258 kDa, 6.131
Gene length, protein length 7002 bp, 2334 aa
Immediate neighbours yxxG, yxxF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
WapA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WapA expression.png















Categories containing this gene/protein

cell wall/ other, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

DegU regulon, YvrHb regulon

The gene

Basic information

  • Locus tag: BSU39230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relexed selection for sporulation PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • the C-terminal toxic domain has RNase activity (cleaves tRNAs) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • cell wall binding domain as retention signal
    • C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed at high salt concentration (DegU-P) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References