Difference between revisions of "RibE"

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(Expression and regulation)
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|style="background:#ABCDEF;" align="center"|'''Function''' || riboflavin biosynthesis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || riboflavin biosynthesis  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU23270 ribE]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23270 ribE]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RibE RibE]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RibE RibE]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ribA]]'', ''[[ribD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ribA]]'', ''[[ribD]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14259&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23270 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ribE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ribE_context.gif]]

Revision as of 13:11, 13 May 2013

  • Description: riboflavin synthase (alpha subunit)

Gene name ribE
Synonyms ribB
Essential no
Product riboflavin synthase (alpha subunit)
Function riboflavin biosynthesis
Gene expression levels in SubtiExpress: ribE
Interactions involving this protein in SubtInteract: RibE
Metabolic function and regulation of this protein in SubtiPathways:
Riboflavin / FAD
MW, pI 23 kDa, 5.845
Gene length, protein length 645 bp, 215 aa
Immediate neighbours ribA, ribD
Sequences Protein DNA Advanced_DNA
Genetic context
RibE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RibE expression.png















Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

FMN-box

The gene

Basic information

  • Locus tag: BSU23270

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1I8D (from E. coli, 36% identity, 59% similarity) PubMed, , 1RVV (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed
  • Additional information:

Biological materials

  • Mutant: GP203 (erm), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References