Difference between revisions of "CdaA"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
(Biological materials)
Line 132: Line 132:
 
** expression of native ''cdaA'' in ''B. subtilis'': pGP1960 (in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 
** expression of native ''cdaA'' in ''B. subtilis'': pGP1960 (in [[pBQ200]]), available in [[Jörg Stülke]]'s lab
 
** expression of ''cdaA''-Strep in ''B. subtilis'' suitable for [[SPINE]]: pGP1986 (in [[pGP382]]), available in [[Jörg Stülke]]'s lab
 
** expression of ''cdaA''-Strep in ''B. subtilis'' suitable for [[SPINE]]: pGP1986 (in [[pGP382]]), available in [[Jörg Stülke]]'s lab
 
* '''FLAG-tag fusion:'''
 
**GP1381 cdaA-3xFLAG ermC (based on [[pGP1966]]), available in [[Jörg Stülke]]'s lab
 
 
          
 
          
 
* '''lacZ fusion:''' GP1339 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab
 
* '''lacZ fusion:''' GP1339 (cat) based on [[pAC6]], available in [[Jörg Stülke]]'s lab
Line 144: Line 141:
 
* '''FLAG-tag construct:'''
 
* '''FLAG-tag construct:'''
 
** GP1331 (''[[cdaA]]''-3xFLAG ''ermC'', based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab
 
** GP1331 (''[[cdaA]]''-3xFLAG ''ermC'', based on [[pGP1087]]), available in [[Jörg Stülke]]'s lab
 +
** GP1381 cdaA-3xFLAG ermC (based on [[pGP1966]]), available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''

Revision as of 13:41, 18 December 2012

  • Description: diadenylate cyclase, synthesis of c-di-AMP in vegetative cells

Gene name cdaA
Synonyms ybbP, ybbQ
Essential no
Product diadenylate cyclase
Function synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaA
Interactions involving this protein in SubtInteract: CdaA
Metabolic function and regulation of this protein in SubtiPathways:
Murein recycling
MW, pI 30 kDa, 8.074
Gene length, protein length 819 bp, 273 aa
Immediate neighbours rsiW, cdaR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbP expression.png















Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01750

Phenotypes of a mutant

  • inactivation of cdaA results in severe beta-lactam sensitivity PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • synthesis of c-di-AMP from two molecules of ATP PubMed
  • Protein family:
  • Paralogous protein(s): CdaS

Extended information on the protein

  • Kinetic information:
  • Domains: contains a DAC domain involved in the synthesis of c-di-AMP PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • the interaction with CdaR stimulates the diadenylate cyclase activity of CdaA PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Additional publications: PubMed