Difference between revisions of "YvcJ"

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|style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes
 
|style="background:#ABCDEF;" align="center"|'''Function''' || required for the expression of late competence genes
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34770 yvcJ]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34770 yvcJ]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 6.702   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 6.702   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcK]]'', ''[[yvcI]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvcK]]'', ''[[yvcI]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15482&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34770 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34770 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34770 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yvcJ_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yvcJ_context.gif]]

Revision as of 13:56, 13 May 2013

  • Description: GTPase, nucleotide-binding protein

Gene name yvcJ
Synonyms
Essential no
Product GTPase
Function required for the expression of late competence genes
Gene expression levels in SubtiExpress: yvcJ
MW, pI 33 kDa, 6.702
Gene length, protein length 885 bp, 295 aa
Immediate neighbours yvcK, yvcI
Sequences Protein DNA Advanced_DNA
Genetic context
YvcJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YvcJ expression.png
























Categories containing this gene/protein

genetic competence

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU34770

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
  • Protein family: UPF0042 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Anne Galinier, University of Marseille, France

Your additional remarks

References

Original publications

Publications on the E. coli homolog