Difference between revisions of "Dxs"

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(Categories containing this gene/protein)
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU24270 dxs]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU24270 dxs]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqxC]]'', ''[[yqiD]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqxC]]'', ''[[yqiD]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14358&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU24270 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU24270 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU24270 Advanced_DNA]
 
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Revision as of 13:15, 13 May 2013

  • Description: 1-deoxyxylulose-5-phosphate synthase

Gene name dxs
Synonyms yqiE
Essential yes PubMed
Product 1-deoxyxylulose-5-phosphate synthase
Function biosynthesis of thiamine
Gene expression levels in SubtiExpress: dxs
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 69 kDa, 6.002
Gene length, protein length 1899 bp, 633 aa
Immediate neighbours yqxC, yqiD
Sequences Protein DNA Advanced_DNA
Genetic context
Dxs context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Dxs expression.png




























Categories containing this gene/protein

biosynthesis of lipids, biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU24270

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2 (according to Swiss-Prot)
  • Protein family: DXPS subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2O1S (from E. coli, 44% identity, 62% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Junfeng Xue, Birgitte K Ahring
Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis.
Appl Environ Microbiol: 2011, 77(7);2399-405
[PubMed:21296950] [WorldCat.org] [DOI] (I p)

Song Xiang, Gerlinde Usunow, Gudrun Lange, Marco Busch, Liang Tong
Crystal structure of 1-deoxy-D-xylulose 5-phosphate synthase, a crucial enzyme for isoprenoids biosynthesis.
J Biol Chem: 2007, 282(4);2676-82
[PubMed:17135236] [WorldCat.org] [DOI] (P p)

Akihiro Sakai, Natsumi Kinoshita, Makoto Kita, Tohoru Katsuragi, Yoshiki Tani
Investigation of 1-deoxy-D-xylulose 5-phosphate synthase and transketolase of Bacillus subtilis in relation to vitamin B6 biosynthesis.
J Nutr Sci Vitaminol (Tokyo): 2003, 49(1);73-5
[PubMed:12882400] [WorldCat.org] [DOI] (P p)