Difference between revisions of "AsnO"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of asparagine
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of asparagine
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU10790 asnO]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10790 asnO]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/aspartate_asparagine.html Asp, Asn]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yisN]]'', ''[[yizA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yisN]]'', ''[[yizA]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12919&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10790 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10790 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10790 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:asnO_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:asnO_context.gif]]

Revision as of 12:30, 13 May 2013

  • Description: asparagine synthase (glutamine-hydrolysing)

Gene name asnO
Synonyms yisO, yucB
Essential no
Product asparagine synthase (glutamine-hydrolysing)
Function biosynthesis of asparagine
Gene expression levels in SubtiExpress: asnO
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 70 kDa, 5.696
Gene length, protein length 1842 bp, 614 aa
Immediate neighbours yisN, yizA
Sequences Protein DNA Advanced_DNA
Genetic context
AsnO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AsnO expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU10790

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate (according to Swiss-Prot)
  • Protein family: glutamine amidotransferase type-2 domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

K Yoshida, Y Fujita, S D Ehrlich
Three asparagine synthetase genes of Bacillus subtilis.
J Bacteriol: 1999, 181(19);6081-91
[PubMed:10498721] [WorldCat.org] [DOI] (P p)