TopB

• Description: DNA topoisomerase III
  
  

Gene name	topB
Synonyms
Essential	no
Product	DNA topoisomerase III
Function	replication
Gene expression levels in SubtiExpress: topB
MW, pI	81 kDa, 9.879
Gene length, protein length	2181 bp, 727 aa
Immediate neighbours	lrpC, ydaJ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, DNA condensation/ segregation

The gene

Basic information

• Locus tag: BSU04260

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to Swiss-Prot)

• Protein family: prokaryotic type I/III topoisomerase family (according to Swiss-Prot)

• Paralogous protein(s): TopA, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P96583

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: topB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)