ydaJ

168

lipoprotein, may modify the extracellular polysaccharide synthesized by YdaL-YdaM-YdaN

Locus: BSU_04270

Molecular weight: 40.84 kDa

Isoelectric point: 4.9

Protein length: 362 aa

Gene length: 1089 bp

Function: unknown

Product: lipoprotein

Essential: no

Synonyms: ydaJ

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 478,944  480,032

The protein

Protein family

• glycosyl hydrolase 8 (GH8) family PubMed

Structure

• 3REN (PDB) (alpha-amylase from Clostridium perfringens, 25% identity) PubMed
• O31486 (AlphaFold)

Localization

• exported with N-terminal signal peptide, attached to the cell membrane (lipoprotein), faces towards the medium PubMed

Expression and Regulation

Operons

• Genes: ydaJ-ydaK-ydaL-ydaM-ydaN

  Description: PubMed

  Regulation

  • expressed during stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C073 (ydaJ::erm), available at the NBRP B. subtilis, Japan
• JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
• BKE04270 (ydaJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGGCAAATCCCCCTTTG,  downstream forward: _UP4_CTGTTAGCCGAAAGGAAGCT
• BKK04270 (ydaJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGGCAAATCCCCCTTTG,  downstream forward: _UP4_CTGTTAGCCGAAAGGAAGCT

References

Reviews

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Original Publications

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