Difference between revisions of "NarI"

From SubtiWiki
Jump to: navigation, search
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || nitrate respiration, nitrogen assimilation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || nitrate respiration, nitrogen assimilation  
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU37250 narI]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37250 narI]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 10.04   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 10.04   
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywiE]]'', ''[[narJ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywiE]]'', ''[[narJ]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15753&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37250 Advanced_DNA]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:narI_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:narI_context.gif]]

Revision as of 14:06, 13 May 2013

  • Description: nitrate reductase (gamma subunit)

Gene name narI
Synonyms
Essential no
Product nitrate reductase (gamma subunit)
Function nitrate respiration, nitrogen assimilation
Gene expression levels in SubtiExpress: narI
MW, pI 25 kDa, 10.04
Gene length, protein length 669 bp, 223 aa
Immediate neighbours ywiE, narJ
Sequences Protein DNA Advanced_DNA
Genetic context
NarI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NarI expression.png
























Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

Fnr regulon

The gene

Basic information

  • Locus tag: BSU37250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (Fnr) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Heike Reents, Richard Münch, Thorben Dammeyer, Dieter Jahn, Elisabeth Härtig
The Fnr regulon of Bacillus subtilis.
J Bacteriol: 2006, 188(3);1103-12
[PubMed:16428414] [WorldCat.org] [DOI] (P p)

M M Nakano, Y P Dailly, P Zuber, D P Clark
Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
J Bacteriol: 1997, 179(21);6749-55
[PubMed:9352926] [WorldCat.org] [DOI] (P p)

H Cruz Ramos, L Boursier, I Moszer, F Kunst, A Danchin, P Glaser
Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms.
EMBO J: 1995, 14(23);5984-94
[PubMed:8846791] [WorldCat.org] [DOI] (P p)