Difference between revisions of "YvcJ"
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* '''Mutant:''' | * '''Mutant:''' | ||
+ | ** 1A885 ( ''yvcJ''::''cat''), {{PubMed|19074378}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC] | ||
* '''Expression vector:''' pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | * '''Expression vector:''' pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab |
Revision as of 09:21, 19 September 2012
- Description: GTPase, nucleotide-binding protein
Gene name | yvcJ |
Synonyms | |
Essential | no |
Product | GTPase |
Function | required for the expression of late competence genes |
Gene expression levels in SubtiExpress: yvcJ | |
MW, pI | 33 kDa, 6.702 |
Gene length, protein length | 885 bp, 295 aa |
Immediate neighbours | yvcK, yvcI |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34770
Phenotypes of a mutant
- reduced genetic competence PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
- Protein family: UPF0042 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium PubMed
Database entries
- Structure:
- UniProt: O06973
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Anne Galinier, University of Marseille, France
Your additional remarks
References
Original publications
Publications on the E. coli homolog
Falk Kalamorz, Birte Reichenbach, Walter März, Bodo Rak, Boris Görke
Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
Mol Microbiol: 2007, 65(6);1518-33
[PubMed:17824929]
[WorldCat.org]
[DOI]
(P p)