Difference between revisions of "YvcJ"
Line 122: | Line 122: | ||
* '''Mutant:''' | * '''Mutant:''' | ||
+ | ** 1A885 ( ''yvcJ''::''cat''), {{PubMed|19074378}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A885&Search=1A885 BGSC] | ||
* '''Expression vector:''' pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab | * '''Expression vector:''' pGP735 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab |
Revision as of 09:21, 19 September 2012
- Description: GTPase, nucleotide-binding protein
Gene name | yvcJ |
Synonyms | |
Essential | no |
Product | GTPase |
Function | required for the expression of late competence genes |
Gene expression levels in SubtiExpress: yvcJ | |
MW, pI | 33 kDa, 6.702 |
Gene length, protein length | 885 bp, 295 aa |
Immediate neighbours | yvcK, yvcI |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34770
Phenotypes of a mutant
- reduced genetic competence PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed
- Protein family: UPF0042 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- can be localized in the cell either as a helical-like pattern or as foci close to the poles and the septa depending on growth phase and on growth medium PubMed
Database entries
- Structure:
- UniProt: O06973
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Regulation: very weak stimuation of expression by citrate and succinate PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
- Antibody:
Labs working on this gene/protein
Anne Galinier, University of Marseille, France
Your additional remarks
References
Original publications
Frédérique Pompeo, Jennifer Luciano, Céline Brochier-Armanet, Anne Galinier
The GTPase function of YvcJ and its subcellular relocalization are dependent on growth conditions in Bacillus subtilis.
J Mol Microbiol Biotechnol: 2011, 20(3);156-67
[PubMed:21709426]
[WorldCat.org]
[DOI]
(I p)
Jennifer Luciano, Elodie Foulquier, Jean-Raphael Fantino, Anne Galinier, Frédérique Pompeo
Characterization of YvcJ, a conserved P-loop-containing protein, and its implication in competence in Bacillus subtilis.
J Bacteriol: 2009, 191(5);1556-64
[PubMed:19074378]
[WorldCat.org]
[DOI]
(I p)
Boris Görke, Elodie Foulquier, Anne Galinier
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway.
Microbiology (Reading): 2005, 151(Pt 11);3777-3791
[PubMed:16272399]
[WorldCat.org]
[DOI]
(P p)
A Galinier, J Haiech, M C Kilhoffer, M Jaquinod, J Stülke, J Deutscher, I Martin-Verstraete
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.
Proc Natl Acad Sci U S A: 1997, 94(16);8439-44
[PubMed:9237995]
[WorldCat.org]
[DOI]
(P p)
Publications on the E. coli homolog