Difference between revisions of "GlgB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || glycogen biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || glycogen biosynthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU30980 glgB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30980 glgB]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 73 kDa, 6.237   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 73 kDa, 6.237   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glgC]]'', ''[[trnB-Glu]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glgC]]'', ''[[trnB-Glu]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15076&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU30980 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU30980 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU30980 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:glgB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:glgB_context.gif]]

Revision as of 13:41, 13 May 2013

  • Description: 1,4-alpha-glucan branching enzyme

Gene name glgB
Synonyms
Essential no
Product 1,4-alpha-glucan branching enzyme
Function glycogen biosynthesis
Gene expression levels in SubtiExpress: glgB
MW, pI 73 kDa, 6.237
Gene length, protein length 1881 bp, 627 aa
Immediate neighbours glgC, trnB-Glu
Sequences Protein DNA Advanced_DNA
Genetic context
GlgB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GlgB expression.png
























Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU30980

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)
  • Paralogous protein(s): AmyX

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

J A Kiel, J M Boels, G Beldman, G Venema
Glycogen in Bacillus subtilis: molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation.
Mol Microbiol: 1994, 11(1);203-18
[PubMed:8145641] [WorldCat.org] [DOI] (P p)