glgA

168

glycogen synthase (ADPGlc)

Locus: BSU_30950

Molecular weight: 55.69 kDa

Isoelectric point: 5.16

Protein length: 484 aa

Gene length: 1455 bp

Function: glycogen biosynthesis

Product: glycogen synthase (ADPGlc)

Essential: no

E.C.: 2.4.1.21

Synonyms: glgA

Genomic Context

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List of homologs in different organisms, belongs to COG0297 (Galperin et al., 2021)

Gene

Coordinates: 3,166,118 → 3,167,572

The protein

Catalyzed reaction/ biological activity

• [(1→4)-α-D-glucosyl]_(n) + ADP-α-D-glucose --> [(1→4)-α-D-glucosyl]_(n+1) + ADP + H⁺ (according to UniProt)

Protein family

• glycosyltransferase 1 family (single member, according to UniProt)

Structure

• 2QZS (PDB) (from E. coli, 37% identity) PubMed
• P39125 (AlphaFold)

Expression and Regulation

Operons

• Genes: glgB-glgC-glgD-glgA-glgP

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE30950 (ΔglgA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCTGACACAGCAAATAATA,  downstream forward: _UP4_GAGCAGGTGACAAGGAGTGG
• BKK30950 (ΔglgA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCTGACACAGCAAATAATA,  downstream forward: _UP4_GAGCAGGTGACAAGGAGTGG

References

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