Difference between revisions of "ArgH"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of arginine  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of arginine  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU29440 argH]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29440 argH]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytzD]]'', ''[[argG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytzD]]'', ''[[argG]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14904&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29440 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:argH_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:argH_context.gif]]

Revision as of 13:36, 13 May 2013

  • Description: argininosuccinate lyase

Gene name argH
Synonyms
Essential no
Product argininosuccinate lyase
Function biosynthesis of arginine
Gene expression levels in SubtiExpress: argH
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 51 kDa, 4.859
Gene length, protein length 1383 bp, 461 aa
Immediate neighbours ytzD, argG
Sequences Protein DNA Advanced_DNA
Genetic context
ArgH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ArgH expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AhrC regulon

The gene

Basic information

  • Locus tag: BSU29440

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-(N(omega)-L-arginino)succinate = fumarate + L-arginine (according to Swiss-Prot)
  • Protein family: Argininosuccinate lyase subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1TJ7 (from Escherichia coli, 47% identity, 64% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed by casamino acids PubMed
    • repressed by arginine (AhrC)
  • Regulatory mechanism:
    • AhrC: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References