Difference between revisions of "NprE"

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|style="background:#ABCDEF;" align="center"|'''Function''' || degradation of proteins  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || degradation of proteins  
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU14700 nprE]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14700 nprE]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 56 kDa, 7.481   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 56 kDa, 7.481   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yktD]]'', ''[[ylaA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yktD]]'', ''[[ylaA]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13343&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14700 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14700 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14700 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:nprE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nprE_context.gif]]

Revision as of 12:44, 13 May 2013

  • Description: extracellular neutral protease B

Gene name nprE
Synonyms
Essential no
Product extracellular neutral protease B
Function degradation of proteins
Gene expression levels in SubtiExpress: nprE
MW, pI 56 kDa, 7.481
Gene length, protein length 1563 bp, 521 aa
Immediate neighbours yktD, ylaA
Sequences Protein DNA Advanced_DNA
Genetic context
NprE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NprE expression.png
























Categories containing this gene/protein

utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

AbrB regulon, ScoC regulon

The gene

Basic information

  • Locus tag: BSU14700

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Similar, but not identical, to that of thermolysin (according to Swiss-Prot)
  • Protein family: peptidase M4 family (according to Swiss-Prot)
  • Paralogous protein(s): NprB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1NPC (of Bacillus cereus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: nprE (according to DBTBS)
  • Sigma factor:
  • Regulation:
    • repressed during exponential growth (ScoC) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

P T Kallio, J E Fagelson, J A Hoch, M A Strauch
The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein.
J Biol Chem: 1991, 266(20);13411-7
[PubMed:1906467] [WorldCat.org] (P p)